Characterization of Arabidopsis Acid Phosphatase Promoter and Regulation of Acid Phosphatase Expression

Characterization of Arabidopsis acid phosphatase promoter and regulation of acid phosphatase expression.

The expression and secretion of acid phosphatase (APase) was investigated in Indian mustard (Brassica juncea L. Czern.) plants using sensitive in vitro and activity gel assays. Phosphorus (P) starvation induced two APases in Indian mustard roots, only one of which was secreted. Northern-blot analysis indicated transcriptional regulation of APase expression. Polymerase chain reaction and Souther...

Functional Assessment of an Overexpressed Arabidopsis Purple Acid Phosphatase Gene (Atpap26) in Tobacco Plants

Background: Overexpression of known genes encoding key phosphate (Pi)-metabolizing enzymes, such as acid phosphatases (APases), is presumed to help plants with Pi availability and absorption as they are mostly exposed to suboptimal environmental conditions for this vital element.Objectives: In this study, the overexpression effect of AtPAP26, one of the m...

Characterization of Acid Phosphatase in the Promastigotes of Three Isolates of Leishmania major

Background: Acid phosphatase (ACP) is suggested to be one of the virulence factors in leishmania and a correlation has been reported between Leishmanial acid phosphatase (ACP) activity and the severity of the disease in all types of leishmaniasis including zoonotic cutaneous leishmaniasis (ZCL), which is a polymorphic disease. Objective: Characterization of leishmania and its correlation with t...

Expression of tartrate-resistant acid phosphatase.

Fungal Infection Alters Phosphate Level and Phosphatase Profiles in Arabidopsis

Phosphorus (P), in the form of phosphate ion (Pi), is a vital element contributing in biomolecule structures, metabolic reactions, signaling pathways and energy transfer within the living cells. The objective of the present study was to assess the influence of fungal infection on Pi metabolism in compare to the effects of phosphate stress in Arabidopsis. Quantification of total P contents showe...